Project A1 - Role of kindlin-3 in hematopoietic cells in vivo
A hallmark of integrins is their ability to shift between inactive and active conformations, a process, which is regulated by a synergistic interaction between two intracellular proteins: talin and kindlins. The proposed project will address the thymus colonization defect of kindlin-3 null mice. Different integrin-mediated processes such as adhesion, signalling and extravasation into inflamed tissues will be studied in kindlin-3 hypomorphic mice, which express very low levels of kindlin-3 in all hematopoietic cells. Finally, posttranslational modifications and novel kindlin-3 interaction partners will be identified by mass spectrometry and further characterized by biochemical and cell biological assays using various blood cells from Flag-tagged kindlin-3 knockin mice.