Project A5 - Proteomics screen for conformation-specific cytoplasmic integrin interactors
Integrins are cell adhesion receptors that are involved in many aspects of haematopoietic cell function. They rely on the recruitment of proteins to their cytoplasmic domains to regulate the conformational switch between a high (‘active’) and a low-affinity (‘inactive’) state for ligands and to initiate intracellular signalling. The proposed project aims at identifying and characterizing proteins that specifically interact with inactive α5β1 and αLβ2 integrins and thereby affect integrin-mediated functions in leukocytes. To this end, we developed a screening strategy that mimics the inactive integrin state.