Advanced Methods Course: Genetic Manipulation in Mice
- June 25 & July 26/27, 2012
26.07.2012
Date:
June 25 (17.15 h) & July 26/27
Topic:
Gene targeting in the mouse is meanwhile a well established technology to study the function of a certain gene in mammals. It uses homologous recombination in mouse embryonic stem (ES) cells to modify mouse genes precisely and allows researchers to create almost any desired modification in the mouse genome ranging from simple knockouts to single amino acid mutations. The lecture will impart basic knowledge on the gene targeting technology itself and what do you have to consider when creating such a mouse.
Since most steps in the manipulation of early mouse embryos require a high degree of experience the practical course is not a typical hands-on course. Nevertheless this course should give you insights into early mouse embryo isolation, ES cell culture and ES cell injection into blastocysts.
Speaker:
Markus Moser, MPI
Host:
Markus Moser, MPI
Location:
Max-Planck-Institut für Biochemie
Am Klopferspitz 18
82152 Martinsried
Note: The number of participants is limited for this course. Registration beforehand is required (irtg914@med.uni-muenchen.de).