Advanced Methods Course: Cell Adhesion and Migration I- May 14/15, 2012
May 14 (17.15 h), May 15 (Full day), 2012
T cell adhesion, extravasation and migration within the tissue are fundamental for an effective adaptive immune response. Leukocyte specific integrins and their ligands are the main adhesion molecules regulating these events. The activity of these integrins on the surface of leukocytes needs to be tightly controlled by multiple signalling cascades. In addition, reorganisation of the cytoskeleton is a prerequisite for leukocyte movement. This lecture will cover the impact of cell adhesion molecules on leukocyte recruitment and will shed light on signalling pathways involved in integrin activation and cytoskeletal rearrangement.
In order identify specific signalling molecules regulating T cell migration downstream or upstream of integrins it is necessary to simplify complex in vivo situations utilising in vitro assays. The use of different assays allows drawing conclusions on different aspects of leukocyte migration (adhesion, migration in 2D vs. 3D, chemotaxis). As such, the choice of an appropriate assay is fundamental. This hands-on course will familiarise participants with different in vitro assays on T cell adhesion and migration and will give advise which assay to use for investigating particular aspects of leukocyte migration.
Melanie Laschinger, Technical University, München
Tanja Weißer, Karin Fütterer & Dr. Melanie Laschinger
Chirurgische Klinik und Poliklinik
Klinikum rechts der Isar
Ismaninger Str. 22
Note: The number of participants is limited for this course. Registration beforehand is required (firstname.lastname@example.org).