Project A08 - Novel mechanisms of immune cell migration revealed by genetic and functional analysis of human primary immune-deficiency disorders
Rare immune defects, especially in children, offer the opportunity to better understand the molecular makeup of cell migration. By using whole exome sequencing it is often possible to identify an underlying genetic cause that impairs immune cell migration. We will identify new informative genes by next generation sequencing (NGS) and subsequently characterize their molecular function. An important experimental setup will be the generation of neutrophils from induced pluripotent stem (iPS) cells using patient fibroblast or genetically engineered cell lines as starting material.