Project A01 - Deciphering mechanisms of kindlin3 and talin1-mediated integrin activation and signaling in hematopoietic cells
Leukocyte trafficking depends on talin1- and kindlin3-mediated control of integrin activity. In the pro-posed project we aim to investigate the role of leupaxin, a new kindlin3 interactor, in adhesion struc-ture formation, signaling and leukocyte trafficking in vitro and in vivo. We further aim to study the molecular mechanisms of talin1 recruitment to the plasma membrane by analyzing mouse mutants and genetically engineered cells carrying mutations in talin1, which abolish different putative pathways of talin1 membrane targeting.